Radiation hybrid mapping of the pig ALDOA, ALDOB and ALDOC genes to SSC3, SSC1 and SSC12.

Source/description: Fructose-1, 6-bisphosphate aldolase is a glycolytic enzyme which plays a critical role in the reversible conversion of fructose-1, 6-bisphosphate to glyceraldehydes 3-phosphate and dihydroxyacetone phosphate. It is reported that in vertebrates fructose-1, 6-bisphosphate aldolase has three isozymes: aldolase A, fructose-bisphosphate (ALDOA); aldolase B, fructose-bisphosphate (ALDOB) and aldolase C, fructose-bisphosphate (ALDOC). In human beings, deficiency of aldolase A has been described as a rare cause of hereditary non-spherocyte haemolytic anaemia (HNSHA); deficiency of aldolase B was involved in hereditary fructose intolerance (HFI), while the potential biological function of aldolase C has not been reported so far. In pig, ALDOB has been previously localized on chromosome 1q18 or 1q28–q213 using somatic cell hybrids, and this location has been narrowed to 1q28– q29 by the fluorescence in situ hybridization. The objective of the present study was to map ALDOA, ALDOB and ALDOC on the porcine radiation hybrid map.